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SRP3031

Sigma-Aldrich

Endostatin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

COL18A1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

mol wt

20.2 kDa

packaging

pkg of 100 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... COL18A1(80781)

General description

Research area: Cell Signaling

Endostatin is an endogenous glycoprotein derived from the C-terminal portion of the α1 chain of type XVIII collagen. It is encoded by the gene mapped to human chromosome 21q22.3. Recombinant human Endostatin protein consists of 184 amino acid residues. It is expressed in various organs with the highest levels in liver, lung, and kidney.

Biochem/physiol Actions

Endostatin is a potent inhibitor of angiogenesis. It exerts its angiostatic effect through multiple pathways related to endothelial cell migration and proliferation. Endostatin inhibits and suppresses the actions of matrix metalloproteinases (MMPs), FAK (Focal adhesion kinase)/Ras/p38-MAPK (mitogen-activated protein kinase)/ERK (extracellular signal-regulated kinase) signaling cascade, HIF-1α(hypoxia-inducible factor 1 alpha)/VEGF-A (vascular endothelial growth factor)thereby inhibiting angiogenesis by preventing the migration and invasion of endothelial cells. Endostatin also blocks the apoptosis of cells and the G1 to S phase transition in the cell cycle. Mutations in this gene lead to Knobloch syndrome (KS), a rare autosomal recessive disorder.

Sequence

MHSHRDFQPV LHLVALNSPL SGGMRGIRGA DFQCFQQARA VGLAGTFRAF LSSRLQDLYS IVRRADRAAV PIVNLKDELL FPSWEALFSG SEGPLKPGAR IFSFDGKDVL RHPTWPQKSV WHGSDPNGRR LTESYCETWR TEAPSATGQA SSLLGGRLLG QSAASCHHAY IVLCIENSFM TASK

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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