Skip to Content
Merck

Skip To

SRP3306

Sigma-Aldrich

SCD40 LIGAND

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

Synonym(s):

CD154, TNFSF5, TRAP, gp39

Sign Into View Organizational & Contract Pricing

Select a Size

100 G
CA$50.00
500 G
CA$162.00

CA$50.00


Please contact Customer Service for Availability

Request a Bulk Order

Select a Size

Change View
100 G
CA$50.00
500 G
CA$162.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.77

CA$50.00


Please contact Customer Service for Availability

Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

5-10 ng/mL ED50

mol wt

16.3 kDa

packaging

pkg of 50 μg

impurities

endotoxin, tested

UniProt accession no.

shipped in

wet ice

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
469701.0388746960
technique(s)

titration: suitable

technique(s)

-

technique(s)

-

technique(s)

titration: suitable

fluorescence

λex 460 nm; λem 515 nm(lit.)

fluorescence

λex 460 nm; λem 515 nm(lit.)

fluorescence

-

fluorescence

λex 460 nm; λem 515 nm(lit.), λex 490 nm; λem 514 nm in 0.1 M Tris pH 8.0

color

orange to brown

color

-

color

red-brown

color

-

solubility

H2O: 1 mg/mL

solubility

-

solubility

500 g/L

solubility

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

application(s)

-

form

powder

form

powder

form

powder

form

powder

General description

CD40, a member of the TNF receptor superfamily, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to “immunodeficiency with hyper-IgM”, a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261 amino acid type II transmembrane protein, which contains a 22 amino acid cytoplasmic domain, a 24 amino acid transmembrane domain, and a 215 amino acid extracellular domain. The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.

Biochem/physiol Actions

CD40, a member of the TNF receptor superfamily, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.

Physical form

Lyophilized from 10mM Sodium Phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

P Kozler et al.
Physiological research, 52(5), 607-614 (2003-10-11)
The aim was to study the blood-brain permeability according to the distribution in the rat brain of Evans blue (EB) and sodium fluorescein (NaFl) administered by an intracarotid injection. Eighteen animals were divided into six groups according to the state
D Montermini et al.
The Journal of physiology, 543(Pt 3), 959-975 (2002-09-17)
The permeability, P(S), to sodium fluorescein (Stokes-Einstein radius = 0.45 nm) has been measured in single mesenteric capillaries of pithed frogs and anaesthetised rats as perfusion velocity, U, was varied over a range from 400 up to 2000-10,000 microm s(-1).
R Oishi et al.
Naunyn-Schmiedeberg's archives of pharmacology, 339(1-2), 159-165 (1989-01-01)
Morphine (5 mg/kg, s.c.) caused a submaximal increase in the brain level of sodium fluorescein administered i.v. Histamine H1-antagonists, diphenhydramine and mepyramine, given either i.p. or i.c.v., had no significant influence on the effect of morphine. H2-Antagonists, cimetidine and ranitidine
In vitro model of the outer blood-retina barrier.
Steuer H
Brain Research. Brain Research Protocols, 13, 26-26 (2004)
Structural mechanisms of acute VEGF effect on microvessel permeability.
American Journal of Physiology. Heart and Circulatory Physiology, 284, H2124-H2124 (2003)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service