SRP8023

PARG human

recombinant, expressed in Sf21 cells, His tagged, >95% (SDS-PAGE)

• Synonym(s): FNDC5 (cleaved), Poly(ADP-ribose) glycohydrolase
• CAS Number: 9068-16-0
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in Sf21 cells
• tag: His tagged
• Assay: >95% (SDS-PAGE)
• form: liquid
• mol wt: ~110 kDa by SDS-PAGE
• packaging: pkg of 2 μg
• concentration: ≥0.2 mg/mL
• technique(s): cell based assay: suitable
• color: clear
• solubility: water: soluble
• UniProt accession no.: Q86W56
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PARG(8505)

Description

General description

Research area: Cell signaling

Application

PARG human has been used in the digestion of sciatic nerve sections, in co-immunoprecipitation, in in vitro glycohydrolase activity assay

Biochem/physiol Actions

Poly(ADP-ribose) glycohydrolase(PARG) hydrolyzes poly(ADP-ribose) at glycosidic(1′′-2′′) linkage of ribose-ribose bond to produce free ADP-ribose. It is an endo- and exo-glycosidase. PARG plays arole in DNA repair and replication. Inhibited or depleted PARG in cells aremore prone to DNA-damaging agents. PARG also averts the accumulation of PAR inthe cytoplasm and parthanatos, a caspase-independent PAR-mediated type of celldeath.

Physical form

Solution in 50 mM TRIS-HCl, pH 7.5, containing 100 mM sodium chloride, 0.2% NP-40, 50 mM imidazole and 10% glycerol.

Other Notes

Human PARG is fused to a His-tag.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H360D
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Repr. 1B
• Storage Class Code: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable