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T1165

Sigma-Aldrich

Tris-Tricine-SDS Buffer 10× Concentrate

Synonym(s):

10X TTS Buffer, 10X Tris Tricine SDS running buffer, Tris Tricine SDS transfer buffer

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1 EA
CA$1,790.00

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1 EA
CA$1,790.00

About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

CA$1,790.00


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sterility

0.2 μm filtered

Quality Level

form

liquid

pH

8.05-8.30 (25 °C in water, high purity, 1:10)

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

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This Item
277746491888491578
mass shift

M+1

mass shift

M+1

mass shift

M+1

mass shift

M+1

isotopic purity

99 atom % 13C

isotopic purity

99 atom % 13C

isotopic purity

99 atom % 13C

isotopic purity

99 atom % 13C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

solid

form

solid

form

solid

form

solid

mp

125-128 °C (lit.)

mp

121-125 °C (lit.)

mp

121-125 °C (lit.)

mp

183-184 °C (lit.)

General description

Tris-Tricine-SDS (TTS) running buffer is the cathode (upper reservoir) buffer for SDS−polyacrylamide gel electrophoresis of proteins using the Schagger and von Jagow method. The Schagger and von Jagow method is designed for the separation of small molecular weight proteins. It differs from the Laemmli method in that the glycine is replaced with tricine and the gel contains 1M Tris-HCl, pH 8.45 instead of 0.375 M Tris-HCl, pH 8.9. Dilution of the 10X TTS buffer produces a 1X cathode buffer containing 100 mM Tris, 100 mM tricine and 0.1% SDS. Recommended running conditions is 125 volts.

Application

Tris-Tricine-SDS Buffer 10× Concentrate is suitable for use as a running buffer for polyacrylamide based separation of bronchoalveolar lavage fluid (BALF).[1]

Other Notes

1M Tris, 1M Tricine, 1% (w/v) SDS, pH approx. 8.2.

Analysis Note

Tested for use as a cathode buffer for Tris-Tricine gel electrophoresis to separate low molecular weight proteins.

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