T7699

Thunderbolt^™ GC10^™ Electrocompetent Cells

for generation of cDNA libraries and DNA plasmid production

• MDL number: MFCD05665590
• UNSPSC Code: 12352200

Properties

• grade: Molecular Biology
• form: suspension
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Thunderbolt GC10 electrocompetent E. coli have a very high transformation efficiency of >1x10¹⁰ cfu/μg when transformed with non-saturating amounts of pUC19 plasmid DNA. They are comparable to DH10β strain.

Application

Suitable for recovery of high quality plasmid DNA and generation of cDNA libraries from plasmid-based vectors

Features and Benefits

• Ensures recovery of stable, high quality plasmid DNA as well as methylated DNA
• Renders protection to clonal stocks from T1 and T5 bacteriophage contamination
• Allows for β-galactosidase α-complementation for blue/white screening
• Guaranteed high transformation efficiency
• Convenient 80 μL or 100 μL aliquots

Components

• Thunderbolt GC10 electrocompetent cells, 5 X 80 μL or 5 X 100 μL (T7074)
• pUC 19 control DNA (10 ng/μL), 10 μL (D2567)

Principle

Thunderbolt GC10 electrocompetent E. coli is K strain bacteria that contain mutations in recA1 and endA1 genes. These mutations aid in minimizing recombination and ensuring plasmid stability. This strain also contains tonA genotype that confers resistance to lytic bacteriophages such as T1 and T5. The host restriction systems are eliminated to allow the cloning of methylated DNA.

Legal Information

GC10 is a trademark of GeneChoice, Inc.

Thunderbolt is a trademark of GeneChoice, Inc.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable