TDF100A
Total Dietary Fiber Assay Kit
sufficient for ~100 assays
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About This Item
UNSPSC Code:
12164500
NACRES:
NA.84
902,00 $
Please contact Customer Service for Availability
Request a Bulk OrderRequest more information
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usage
sufficient for ~100 assays
Quality Level
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
1 of 4
This Item | AXYEV50R | AXYTF400RS | AXYTF300RS |
|---|---|---|---|
| manufacturer/tradename Axygen Inc EVF-50-R-S, Corning | manufacturer/tradename Axygen Inc EV-50-R, Corning | manufacturer/tradename Axygen Inc TF-400-R-S, Corning | manufacturer/tradename Axygen Inc TF-300-R-S, Corning |
| sterility sterile | sterility non-sterile | sterility sterile | sterility sterile |
| packaging pkg of 5x960tips/cs | packaging pkg of 5x960tips/cs | packaging pkg of 5x960tips/cs | packaging pkg of 5x960tips/cs |
| capacity 50 μL | capacity 50 μL | capacity - | capacity - |
| color clear | color clear | color - | color - |
Application
This assay determines the total dietary fiber content of food using a combination of enzymatic and gravimetric methods. Samples of dried, fat-free foods are gelatinized with heat stable α-amylase and then enzymatically digested with protease and amyloglucosidase to remove the protein and starch present in the sample. Ethanol is added to precipitate the soluble dietary fiber. The residue is then filtered and washed with ethanol and acetone. After drying, the residue is weighed. Half of the samples are analyzed for protein and the others are ashed. Total dietary fiber is the weight of the residue less the weight of the protein and ash.
Other Notes
Reagents for the determination of total dietary fiber.
Principle
Uses a combination of enzymatic and gravimetric methods. This procedure is based on the method published in Official Methods of Analysis, 15th ed., AOAC, Arlington, VA, Vol. II, Sec. 985.29, 1105 (1990).
Kit Components Only
Product No.
Description
- α-Amylase, Heat-stable 10 mL
- Amyloglucosidase 10 mL
- Celite 50 g
- Protease 500 mg
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
Target Organs
Lungs, Respiratory system
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Federica Pellati et al.
Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)
In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts
Milan Nobilis et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(32), 3845-3852 (2011-11-22)
New bioanalytical SPE-HPLC-PDA-FL method for the determination of the neuroleptic drug tiapride and its N-desethyl metabolite was developed, validated and applied to xenobiochemical and pharmacokinetic studies in humans and animals. The sample preparation process involved solid-phase extraction of diluted plasma
Hiroyuki Kataoka et al.
Analytical and bioanalytical chemistry, 405(1), 331-340 (2012-10-16)
We have developed a simple and sensitive method for the simultaneous determination of testosterone (TES), cortisol (CRT), and dehydroepiandrosterone (DHEA) in saliva by automated online in-tube solid-phase microextraction (SPME) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a Discovery HS
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
Ilia Brondz et al.
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein
Chromatograms
application for SPE, application for HPLCOur team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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