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ZRB1219

Sigma-Aldrich

Anti-MBP Antibody, clone 2N6 , ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):

Myelin A1 protein, Myelin basic protein, Myelin membrane encephalitogenic protein

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100 MG
CA$71.90

About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

CA$71.90


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biological source

rabbit (recombinant)

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

2N6, recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 17.34 kDa
calculated mol wt 33.11 kDa
observed mol wt 18-20 kDa

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species reactivity

mouse, rat, human

species reactivity

human, mouse

species reactivity

rat, human

species reactivity

human, mouse, rat

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

clone

2N6, recombinant monoclonal

clone

1F22, monoclonal, recombinant monoclonal

clone

2B21, monoclonal, recombinant monoclonal

clone

1G7, monoclonal, recombinant monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

Gene Information

human ... MBP(4155)
mouse ... Mbp(17196)
rat ... Mbp(24547)

Gene Information

human ... MYOD1(4654)

Gene Information

human ... SYP(6855)

Gene Information

human ... SYP(6855)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 2N6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Myelin Basic Protein (MBP). It targets an epitope within 19 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human Myelin Basic Protein.

Application

Anti-MBP, clone 2N6 ZooMAb, Cat. No. ZRB1219, is a highly specific recombinant rabbit monoclonal antibody that detects Myelin basic protein and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MBP in mouse and rat brain tissue lysate.

Immunocytochemistry Analysis: A 1:10 dilution from a representative lot detected MBP in Rat Glial precursor cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MBP in human brain and mouse brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens and experimental conditions may vary.

Target description

Myelin basic protein (UniProt: P02686; also known as MBP, Myelin A1 protein, Myelin membrane encephalitogenic protein) is encoded by the MBP gene (Gene ID: 4155) in human. MBP is a homodimeric protein that is found in both the central and the peripheral nervous system. It is one of the most abundant protein components of the myelin membrane in the CNS and plays a role in both the formation and stabilization of this myelin membrane. It is responsible for adhesion of the cytosolic surfaces of multi-layered compact myelin. MBP can interact with many polyanionic proteins including actin, tubulin, calmodulin, and clathrin, and with negatively charged lipids. MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system. MBP is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. MBP expression begins abruptly in 14-16-week-old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane that can lead to demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating Marburg disease. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Haisu Shi et al.
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Meat science, 157, 107872-107872 (2019-07-06)
This study aims to investigate the effect of algae supplementation in high-energy diet (HE diet) on lipid metabolism of intensive feeding sheep. The lambs were assigned to two groups and received a standard diet (ST diet, 8.40 MJ/kg) or a HE
Effect of microwave cooking or broiling on selected nutrient contents, fatty acid patterns and true retention values in separable lean from lamb rib-loins, with emphasis on conjugated linoleic acid
Maranesi.M, et al.
Food Chemistry, 90, 207-218 (2005)
Ronan Lordan et al.
Foods (Basel, Switzerland), 8(5) (2019-05-30)
The in vitro antithrombotic properties of polar lipid constituents of malted grain (MG), pelleted hops (PH), brewer's spent grain (BSG), spent hops (SH), wort, and bottled beer from the same production line were assessed in human platelets. The total lipids
Effect of microwave cooking or broiling on selected nutrient contents, fatty acid patterns and true retention values in separable lean from lamb rib-loins, with emphasis on conjugated linoleic acid
Maranesi.M, et al.
Food Chemistry, 90(1-2), 207-218 (2005)

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