50631-U

Ascentis^® Express 90 Å F5 (5 μm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• Synonym(s): Core-shell (SPP) Fused Core PFP HPLC column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• Product Name: Ascentis^® Express F5, 5 μm HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L43
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• parameter: 60 °C temp. range; 600 bar max. pressure (9000 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 15 cm × 4.6 mm
• surface area: 90 m²/g
• impurities: <5 ppm metals
• matrix: Fused-Core particle platform; superficially porous particle
• matrix active group: PFP (pentafluorophenyl) phase
• particle size: 5 μm
• pore size: 90 Å pore size
• operating pH: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

Ascentis^® Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core^® particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Application

• Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study employed the Ascentis^® Express F5, 5 µm HPLC column to determine lumacaftor and its degradation products in the presence of ivacaftor. The application of high-performance liquid chromatography ion trap time-of-flight mass spectrometry provided high sensitivity and resolution, showcasing the column′s efficacy in complex sample matrices (Özcan et al., 2023).


• Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: This research highlights the use of the Ascentis^® Express F5, 5 µm HPLC column in developing stability-indicating methods for tasimelteon. The column′s performance in both LC-MS/MS and LC-DAD methods ensured accurate and reliable identification of degradation products (Özcan et al., 2020).


• Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review discusses advancements in HPLC technology, including the Ascentis^® Express F5, 5 µm HPLC column. The article emphasizes the column′s capability in ultra-fast separations, providing enhanced efficiency and resolution in various analytical applications (Ali et al., 2012).

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H314
• Precautionary Statements: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1B
• Storage Class Code: 8B - Non-combustible corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable