50632-U

Ascentis^® Express 90 Å F5 (5 μm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

• Synonym(s): Core-shell (SPP) Fused Core PFP HPLC column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• Product Name: Ascentis^® Express F5, 5 μm HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L43
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• parameter: 60 °C temp. range; 600 bar max. pressure (9000 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 25 cm × 4.6 mm
• surface area: 90 m²/g
• impurities: <5 ppm metals
• matrix: Fused-Core particle platform; superficially porous particle
• matrix active group: PFP (pentafluorophenyl) phase
• particle size: 5 μm
• pore size: 90 Å pore size
• operating pH: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

Ascentis^® Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core^® particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Application

• Liquid chromatographic determination of lumacaftor in the presence of ivacaftor and identification of five novel degradation products using high-performance liquid chromatography ion trap time-of-flight mass spectrometry.: This study employs an Ascentis^® Express F5, 5 µm HPLC column for the precise determination of lumacaftor amidst ivacaftor and identifies novel degradation products, showcasing the column′s efficacy in complex mixture analysis (Özcan et al., 2023).


• Stability-indicating LC-MS/MS and LC-DAD methods for robust determination of tasimelteon and high resolution mass spectrometric identification of a novel degradation product.: Utilizing the Ascentis^® Express F5, 5 µm HPLC column, this research developed stability-indicating methods for tasimelteon, highlighting the column′s robustness in pharmaceutical analysis (Özcan et al., 2020).


• Recent trends in ultra-fast HPLC: new generation superficially porous silica columns.: This review explores the advancements in HPLC columns, including the Ascentis^® Express F5, emphasizing its role in achieving ultra-fast separations in analytical chemistry (Ali et al., 2012).

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable