G004597
HPLC Analysis of Metoprolol Enantiomers on Astec® CHIROBIOTIC® T2
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: CHIROBIOTIC T2, 25 cm x 4.6 mm I.D., 5 μm particles (16024AST)
mobile phase: 0.1 wt% ammonium trifluoroacetic acid in methanol
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 230 nm
injection: 5 μL
sample: 1 mg/mL in methanol
suitability
application for HPLC
application(s)
pharmaceutical (small molecule)
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC
Analyte
Description
(±)-Metoprolol (+)-tartrate salt, ≥98% (titration), powder
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