164545

7-Amino-4-methylcoumarin

≥98% (HPLC), solid

• Synonym(s): 7-Amino-4-methylcoumarin, AMC, Coumarin 120
• Empirical Formula (Hill Notation): C₁₀H₉NO₂
• CAS Number: 26093-31-2
• Molecular Weight: 175.18
• MDL number: MFCD00006868
• UNSPSC Code: 12352200
• NACRES: NA.21

Properties

• Product Name: 7-Amino-4-methylcoumarin, Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity.
• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: yellow
• solubility: DMSO: 10 mg/mL; acetone: 10 mg/mL; DMF: soluble
• fluorescence: λ_ex 365-380 nm; λ_em 430-460 nm
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: Nc1cc2c(cc1)C(=CC(=O)O2)C
• InChI: 1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3
• InChI key: GLNDAGDHSLMOKX-UHFFFAOYSA-N

Description

General description

Reagent used to prepare fluorogenic 7-amido-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzyme activity. Useful as a reference standard in enzyme assays.

Reagent used to prepare fluorogenic 7-amino-4-methylcoumarin (AMC) based substrates for the detection of proteolytic enzymes (excitation maximum: ~365-380 nm; emission maximum: ~430-460 nm). Also useful as a reference standard in enzyme assays.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Detection of proteolytic enzyme activity

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). DMF and DMSO stock solutions are stable for up to 6 months at -20°C. Acetone stock solutions are stable for up to 1 week at 4°C or -20°C.

Other Notes

Kanaoka, Y., et al. 1982. Chem. Pharm. Bull. 30, 1485.
Zimmerman, M., et al. 1977. Anal. Biochem. 78, 47.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable