R7647

Random Nonamers

for use as primers in cDNA synthesis, 50 μM in H₂O

• Synonym(s): Oligodeoxynucleotides
• UNSPSC Code: 41105500
• NACRES: NA.31

Properties

• grade: Molecular Biology
• Quality Level: 200
• form: liquid
• usage: 0.1 mL sufficient for 100 RT-PCR reactions
• concentration: 50 μM in H₂O
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Random Nonamers are random sequences of nine deoxyribonucleotides (9-mers) used to prime mRNA, with or without a poly(A) tail. Random nonamers may be used as universal, non-specific primers.

Application

Random Nonamers are suitable for:

• First-strand cDNA synthesis
• Preparing cDNA libraries (with oligo (dT) primers
• Prepare labeled DNA probes for hybridizations

Features and Benefits

• When preparing cDNA libraries or dealing with situations where specific primers are not suitable, the random nonamers can be combined with anchored oligo (dT)23 primers (Product No. O4387).
• In cases of incomplete or missing sequence information, or when specific primers are not effective, the random nonamers offer a solution for cDNA library preparation.
• The random nonamers′ priming capability at higher temperatures (up to 65°C) is reduced, ensuring that they do not interfere with polymerase chain reaction(PCR) following transcription.
• Randomnonamers can also be used to prepare labeled DNA probes for hybridizationstudiesRandom nonamers serve as alternatives to specific reverse transcription (RT) primers for first-strand synthesis, cDNA library construction, and other applications.

Components

Random Nonamers are provided as a 50 uM solution in water.

Other Notes

For laboratory use only. Not for drug, household, or other uses.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves