17-700
Zestaw do immunoprecypitacji białek wiążących RNA Magna RIP®
RNA Immunoprecipitation (RIP) Kit containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions using protein A/G magnetic beads.
Synonim(y):
Immunoprecypitacja RNA, Immunoprecypitacja RNA za pomocą kulek magnetycznych, Magnetyczny zestaw RIP, Zestaw RIP
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.32
Pomoc techniczna
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Pozwól nam pomócPomoc techniczna
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Pozwól nam pomócPoziom jakości
producent / nazwa handlowa
Magna RIP®
Upstate®
metody
RIP: suitable
immunoprecipitation (IP): suitable
Warunki transportu
dry ice
Powiązane kategorie
Opis ogólny
Gene regulation plays a critical role in complex cellular processes such as development, differentiation, and cellular response to environmental changes. In addition to transcriptional regulation of gene expression by transcription factors, cells utilize post-transcriptional regulatory mechanisms. One such mechanism involves use of certain RNA-binding proteins (RBPs) to temporally and coordinately regulate the rate of mRNA translation of functionally related gene
products. While the regulation of gene expression by transcription factors has been well studied over time, the post-transcriptional regulation of mRNAs by RBPs and the role of non-coding RNAs in this process is a relatively nascent field that remains to be thoroughly explored.
products. While the regulation of gene expression by transcription factors has been well studied over time, the post-transcriptional regulation of mRNAs by RBPs and the role of non-coding RNAs in this process is a relatively nascent field that remains to be thoroughly explored.
RNA-binding protein immunoprecipitation (RIP) is the RNA analog of the more well-known ChIP application (chromatin immunoprecipitation), which identifies DNA targets of DNA-binding proteins in an in-vivo cellular context. RIP can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins. These experiments involve immunoprecipitation of endogenously formed complexes of RNA-binding proteins and co-isolation of any RNA species associated with that RNA-binding protein. Purification of these RNA species allows interrogation and identification of mRNAs (and potentially non-coding RNAs associated with them) and can be directly measured using down stream applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and “deep-sequencing” or 2nd-generation sequencing based platforms (RIP-Seq).
Features & Benefits:
-Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
-RNAse inhibitors and RNAse-free reagents
-Negative controls
Features & Benefits:
-Protein A/G magnetic beads, optimized to bind nucleic acid-protein immune complexes
-RNAse inhibitors and RNAse-free reagents
-Negative controls
Zastosowanie
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Opakowanie
RIP Kit capacity: 12 RNA-binding protein immunoprecipitation assays
Komponenty
Magnetic Beads Protein A/G
RIP Wash Buffer
RIP Lysis Buffer
0.5 M EDTA
10% SDS
Salt Solution I
Salt Solution II
Precipitate Enhancer
Normal Mouse IgG
Rabbit IgG Purified
Protease Inhibitor Cocktail 200X
RNase Inhibitor
Proteinase K (10 mg/mL)
Nuclease free water
RIP Wash Buffer
RIP Lysis Buffer
0.5 M EDTA
10% SDS
Salt Solution I
Salt Solution II
Precipitate Enhancer
Normal Mouse IgG
Rabbit IgG Purified
Protease Inhibitor Cocktail 200X
RNase Inhibitor
Proteinase K (10 mg/mL)
Nuclease free water
Postać fizyczna
Two boxes containing all necessary reagents to perform 12 individual RNA-binding protein immunoprecipitation (RIP) reactions.
Przechowywanie i stabilność
Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed.
Informacje prawne
MAGNA RIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2
Kod klasy składowania
10 - Combustible liquids
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