SF016

ESGRO-2i Medium, 100ml

to enhance viability of mouse embryonic stem and iPS cells

• Synonim(y): 2i Naive ES/IPS Media
• Kod UNSPSC: 12352207
• eCl@ss: 32160801
• NACRES: NA.81

Właściwości

• Poziom jakości: 100
• Formularz: liquid
• metody: cell culture | stem cell: suitable
• moc wejściowa: sample type: mouse embryonic stem cell(s); sample type induced pluripotent stem cell(s)

Opis

Opis ogólny

Two stages of mouse pluripotency have been stabilized to date through in vitro culture. ‘Naive’ represents the earliest stage, which can be enriched for a purer population, termed ‘ground state’. Naive most closely approximates embryonic epiblast in the preimplantation blastocyst. The primed state most closely approximates in vivo immediate postimplantation epiblast. There are clear differences between these stages in culture, expression, epigenetics (including appropriate X-inactivation) and developmental competence. The fact that naive mESCs can generate whole animals through tetraploid complementation while primed mESCs cannot, is a particularly compelling reason for heightened expectations from naive hESCs.
ESGRO-2i medium is a defined, LIF containing medium provided with a selective GSK3β and Mek 1/2 inhibitors to enhance viability of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for cell expansion and growth at clonal density in serum-free conditions and supports a naive/ground pluripotent state.

Zastosowanie

Suitable for ES and iPS cell derivation, passaging and maintenance. Media can be used on feeder dependent and independent pluirpotent stem cell lines.

Important passaging information
Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures.
Recommended products include:
ESGRO Complete^™ Accutase (Cat # SF006)
ESGRO Complete Trypsin Solution (proprietary) (Cat # SF007)
ESGRO Complete Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)

Uwaga dotycząca przygotowania

1. ESGRO-2i Basal Medium with LIF: (Nr katalogowy CS204463) Jedna (1) butelka o pojemności 100 ml zawierająca pożywkę podstawową uzupełnioną LIF, pH 7,1-7,3.

2. Suplement inhibitora GSK3β: (nr katalogowy SF012-050) Jedna (1) fiolka o pojemności 50 μl zawierająca suplement selektywnego inhibitora GSK3β.

3. Suplement inhibitora Mek1/2: (nr katalogowy CS204465) Jedna (1) fiolka 10 μL zawierająca suplement selektywnego inhibitora Mek 1/2.

Przechowywanie i stabilność

• Upon receipt, store basal medium and inhibitor supplements in the dark at -20°C until use. Refer to lot expiration date on labels.
• After basal medium and inhibitor supplements are combined, store the complete ESGRO-2i medium in the dark at 4°C for up to 10 days. Do not refreeze.

Informacje prawne

cOmplete is a trademark of Roche

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids