CSPD-RO
Roche
CSPD ready-to-use
Disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo [3.3.1.13,7]decan}-4-yl)phenyl phosphate
Synonim(y):
ready-to-use, cspd
Pomoc techniczna
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Próba
>98% (HPLC)
Formularz
solution
masa cząsteczkowa
Mr 461
opakowanie
pkg of 1 mL (11655884001[25mM])
pkg of 2 × 50 mL (11755633001[0.25mM] ready-to-use)
producent / nazwa handlowa
Roche
temp. przechowywania
2-8°C
Opis ogólny
CSPD is a chemiluminescent substrate for alkaline phosphatase that enables extremely fast and sensitive detection of biomolecules by producing visible light. Light emission is recorded on X-ray film or on luminescence imager systems.
Zastosowanie
CSPD ready-to-use has been used for:
- the nonradioactive detection of labeled nucleic acid probes on membrane blots (Southern blot, Northern blot, Western blot and Dot blot)
- colony and plaque hybridization
- gel shift assay
- sequencing
Cechy i korzyści
- Save time with a fast, highly sensitive substrate.
- Easily strip and reprobe membranes.
- Take multiple exposures of up to two days for each experiment.
- Reuse the substrate by filtering and storing in sodium azide.
Postać fizyczna
25mM solution (11.6mg/ml), 100x concentrated, colorless solution.
Uwaga dotycząca przygotowania
Storage conditions (working solution): Washing Buffer: 15 to 25 °C
Maleic Acid Buffer: 15 to 25 °C
Detection Buffer: 15 to 25 °C
Blocking Stock Solution, 10x: 2 to 8 °C
Blocking Solution: always prepare fresh
Antibody Solution: 2 to 8 °C
Maleic Acid Buffer: 15 to 25 °C
Detection Buffer: 15 to 25 °C
Blocking Stock Solution, 10x: 2 to 8 °C
Blocking Solution: always prepare fresh
Antibody Solution: 2 to 8 °C
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
Purity: Purified by HPLC
Development time: Variable
Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.
Activated by preincubation (10 minutes at +37°C).
Apply substrate quickly to avoid drying of membrane (background).
Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
Do not use nitrocellulose membranes.
Repeat exposures can be made several days after addition of substrate.
Purity: Purified by HPLC
Development time: Variable
Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.
Activated by preincubation (10 minutes at +37°C).
Apply substrate quickly to avoid drying of membrane (background).
Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
Do not use nitrocellulose membranes.
Repeat exposures can be made several days after addition of substrate.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°C)
does not flash
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