VP002

VirusExpress^® 293 AAV Production Cells

• Synonim(y): HEK293 cells for AAV production
• Kod UNSPSC: 12352200

Właściwości

• Poziom jakości: 600
• Warunki transportu: liquid nitrogen
• temp. przechowywania: −196°C

Opis

Opis ogólny

VirusExpress^® 293 AAV Production Cells are a clonal, suspension-adapted HEK293 derivative selected for fast growth, high transfection efficiency, and high-titer AAV production. VirusExpress 293 AAV Production Cells grow to high cell densities and are amenable to chemical-based transfection reagents commonly used in manufacturing (i.e., PEI, lipid-nanoparticle). A medium exchange step is not needed prior to transfection, and the cells can be transfected in larger volumes, enabling scale-up of AAV production. The cells are supplied in a chemically defined media formulation and can be thawed directly into EX-CELL^® CD HEK293 Viral Vector Medium.

VirusExpress 293 AAV Production Cells are derived from the HEK293 cell line (ATCC CRL-1573) through single cell cloning. The parental HEK293 cell line was established by transformation of human embryonic kidney cells with sheared human adenovirus 5 DNA (Graham F.L. et al. 1977). A 4-kb adenoviral genome fragment is known to have integrated into chromosome 19 and encodes for E1A/E1B proteins. Our clonal derivative eliminates variability from the population while also being adapted to suspension, serum-free conditions. Suspension culture and chemically defined medium allow scalability into stirred tank bioreactors for yields that can meet commercial needs.

Opakowanie

This product is shipped in a liquid nitrogen dry vapor shipper. The liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt.

Inne uwagi

The cells are supplied in a vial containing 1 mL of cells at 1 x 10⁷ viable cells/mL. The cells were banked under cGMP conditions in chemically defined medium and 8% DMSO.

Informacje prawne

A commercial license must be taken prior to use of the cells, or any products or materials derived from the cells, in a manufacturing process. Please contact your local sales representative for more details.

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable