11191

Histopaque^®-1119

sterile-filtered, density: 1.119

• Kod UNSPSC: 12352207
• NACRES: NA.75

Właściwości

• sterylność: sterile-filtered
• Poziom jakości: 400
• Formularz: liquid
• okres trwałości: Expiry date on the label.
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: endotoxin, tested
• gęstość: 1.119
• Zastosowanie: hematology; histology
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Histopaque^®-1119 is a sterile, endotoxin tested, ready-to-use medium. This solution consists of polysucrose and sodium diatrizoate, adjusted to a relative density of 1.119 g/mL and pH 8.8–9.0.

Zastosowanie

Histopaque^®-1119 is suitable for use in conjunction with Histopaque-1077 as it facilitates the rapid recovery of viable mononuclear cells and granulocytes from small volumes of whole blood or bone marrow

Cechy i korzyści

Histopaque^®-1119 offers numerous advantages, such as:

• Optimal recovery of viable cells
• Permits selective separation of blood cell types
• Prevents cell distortion
• Preserves cell viability
• Minimal extraneous cell interference
• Reproducible performance from batch to batch
• Sterile-filtered and endotoxin-tested
• Stable at 2-8°C for at least 2 years when protected from light

Zasada

The process involves the formation of a double gradient on layering an equal volume of Histopaque-1077 over Histopaque-1119. Anticoagulated whole blood is carefully layered onto the upper Histopaque-1077 medium. Upon centrifugation, the erythrocytes are aggregated by polysucrose and thus rapidly sediment. Granulocytes are found at the lower Histopaque-1077/1119 interface; whereas, lymphocytes and other mononuclear cells are found at the upper plasma/Histopaque-1077 interface. Most extraneous platelets found in the upper interface are removed by low-speed centrifugation during washing steps. Erythrocyte contamination is negligible.

Informacje prawne

Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS05
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H290,H315,H319
• Zwroty wskazujące środki ostrożności: P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Klasyfikacja zagrożeń: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
• Kod klasy składowania: 8A - Combustible corrosive hazardous materials
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable