59304

2-Propanol

≥99.5% (GC), for molecular biology, BioUltra

• Synonim(y): sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol
• Wzór liniowy: (CH₃)₂CHOH
• CAS Number: 67-63-0
• Masa cząsteczkowa: 60.10
• Beilstein: 635639
• Numer WE: 200-661-7
• Numer MDL: MFCD00011674
• Kod UNSPSC: 12352104
• Identyfikator substancji w PubChem: 57651672
• NACRES: NA.25
• Bp: 82 °C (lit.)

Właściwości

• Product Name: 2-Propanol, BioUltra, Molecular Biology, ≥99.5% (GC)
• klasa czystości: Molecular Biology
• Poziom jakości: 200
• gęstość pary: 2.1 (vs air)
• ciśnienie pary: 33 mmHg ( 20 °C); 44 mmHg ( 25 °C)
• linia produktu: BioUltra
• Próba: ≥99.5% (GC)
• Formularz: liquid
• temp. samozapłonu: 750 °F
• granice wybuchowości: 2.0-12.7 %, 93 °C
• metody: DNA extraction: suitable; RNA extraction: suitable
• zanieczyszczenia: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pozostałość po odparowaniu: ≤0.001%
• kolor: colorless
• współczynnik refrakcji: n20/D 1.377 (lit.); n20/D 1.378
• bp: 82 °C (lit.)
• mp: −89.5 °C (lit.)
• rozpuszczalność: water: soluble (completely)
• gęstość: 0.785 g/mL at 25 °C (lit.)
• ślady kationów: Al: ≤0.5 mg/kg; Ba: ≤0.1 mg/kg; Bi: ≤0.1 mg/kg; Ca: ≤0.5 mg/kg; Cd: ≤0.05 mg/kg; Co: ≤0.02 mg/kg; Cr: ≤0.02 mg/kg; Cu: ≤0.02 mg/kg; Fe: ≤0.1 mg/kg; K: ≤0.5 mg/kg; Li: ≤0.1 mg/kg; Mg: ≤0.1 mg/kg; Mn: ≤0.02 mg/kg; Mo: ≤0.1 mg/kg; Na: ≤0.5 mg/kg; Ni: ≤0.02 mg/kg; Pb: ≤0.1 mg/kg; Sr: ≤0.1 mg/kg; Zn: ≤0.1 mg/kg
• ciąg SMILES: CC(C)O
• λ: neat
• absorpcja UV: λ: 260 nm A_max: 0.02; λ: 280 nm A_max: 0.01
• przydatność: suitable for molecular biology
• InChI: 1S/C3H8O/c1-3(2)4/h3-4H,1-2H3
• Klucz InChI: KFZMGEQAYNKOFK-UHFFFAOYSA-N

Opis

Opis ogólny

2-propanol is a clear, colorless, polar organic solvent commonly used in chemical and biochemical laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. Isopropanol is commonly used in molecular biology for DNA and RNA extraction through precipitation. Unlike ethanol, which requires 2.5 times the volume of the nucleic acid-containing sample, isopropanol can be added in an equal volume. This causes precipitation to occur even without cooling, making it advantageous in reducing the overall volume of the sample. Moreover, it has applications in quantifying hydroxyproline levels, assessing cell viability through MTT assays, and contributing to microarray hybridization processes.

Zastosowanie

2-Propanol has been used:

• to precipitate genomic DNA extracted from flies
• to precipitate genomic DNA extracted from xenograft primary tumors and organs
• to immunoprecipitate 5-bromo-2-deoxyuridine (BrdU)

Działania biochem./fizjol.

Isopropanol (2-Propanol) is widely used in molecular biology for the precipitation of DNA during extraction from cells and tissues. BioUltra IPA is suitable for DNA precipitation.

Cechy i korzyści

• Suitable as a reagent for Biochemical and Molecular Biology Research
• BioUltra grade, meets strict purity standards through rigorous testing
• Free from DNase, NICKase, RNase, and Protease
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Inne uwagi

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H225,H319,H336
• Zwroty wskazujące środki ostrożności: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
• Organy docelowe: Central nervous system
• Kod klasy składowania: 3 - Flammable liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): 53.6 °F - closed cup
• Temperatura zapłonu (°C): 12.0 °C - closed cup
• Środki ochrony indywidualnej: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter