C2139

Collagenase from Clostridium histolyticum

Name: Collagenase from <I>Clostridium histolyticum</I>
Brand: SIGMA

suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV), Type VIII, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Synonim(y):

Clostridiopeptidase A

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About This Item

CAS Number:

9001-12-1

Numer WE:

3.4.24.3 (BRENDA, IUBMB)

Numer WE:

232-582-9

Numer MDL:

MFCD00130830

Kod UNSPSC:

12352204

NACRES:

NA.54

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pochodzenie biologiczne

Clostridium histolyticum

Poziom jakości

200

typ

Type VIII

Formularz

powder

aktywność właściwa

≥125 CDU/mg solid
0.5-5.0 FALGPA units/mg solid

masa cząsteczkowa

68-125 kDa

przydatność

suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV)

Zastosowanie

diagnostic assay manufacturing

temp. przechowywania

−20°C

Opis ogólny

Clostridium histolyticum is a pathogenic clostridium that produces crude collagenases. This is a mixture of enzymes containing collagenase, non-specific proteases and clostripain.

Zastosowanie

Collagenase may be used:

for the preparation of arterial tissue for the study of advanced glycosylation end products (AGE)
for use along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues.
in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes.
for the preparation of viable hepatocytes from rat liver and for the isolation of fat cells from rat adipose tissue

Działania biochem./fizjol.

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(β-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, β-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline. Collagenase enzymes and neutral protease plays an important role in the effective release of cells from tissue. Collagenase recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X represents a neutral amino acid.

Definicja jednostki

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.