C7902

Calcium chloride dihydrate

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

• Wzór empiryczny (zapis Hilla): CaCl₂ · 2H₂O
• CAS Number: 10035-04-8
• Masa cząsteczkowa: 147.01
• Numer WE: 233-140-8
• Numer MDL: MFCD00149613
• Kod UNSPSC: 12352302
• Identyfikator substancji w PubChem: 24892994
• NACRES: NA.71

Właściwości

• linia produktu: BioReagent
• Poziom jakości: 200
• Próba: ≥99.0%
• Formularz: powder
• metody: cell culture | insect: suitable; cell culture | mammalian: suitable; cell culture | plant: suitable
• mp: 176 °C (dec.) (lit.)
• rozpuszczalność: H₂O: 294 mg/mL, clear, colorless
• ciąg SMILES: O.O.Cl[Ca]Cl
• InChI: 1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2
• Klucz InChI: LLSDKQJKOVVTOJ-UHFFFAOYSA-L

Opis

Opis ogólny

Calcium chloride (CaCl₂) is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. CaCl₂ is used in the preparation and transformation of competent Escherichia coli (E. coli) and the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl₂-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. CaCl₂ is used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from E. coli. The crystallization of porcine pancreatic elastase in the presence of CaCl₂ along with sodium citrate reveals the binding of calcium in the metal-binding site of the protein. The effect of CaCl₂ on the total fluorescence in the polymerization of the tubulin-like filamenting temperature-sensitive mutant Z (FtsZ) division protein of E. coli has been studied by a multiwell fluorescent assay.

Zastosowanie

Calcium chloride dihydrate has been used:

• in the formulation of media
• in sperm collection and preparation
• in cell culture and protoplast preparation

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H319
• Zwroty wskazujące środki ostrożności: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Klasyfikacja zagrożeń: Eye Irrit. 2
• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves