A549 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012804, human lung (carcinoma), Epithelial

• Synonim(y): Komórki A-549, Komórki A549, Komórki NCI-A549
• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: human lung (carcinoma)
• opakowanie: tube of 5 μg 86012804-DNA-5UG; pkg of vial of cells 86012804-1VL
• tryb wzrostu: Adherent
• kariotyp: Hypotriploid
• morfologia: Epithelial
• produkty: Lecithin, High amounts of de-saturated fatty acids.
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Caucasian lung carcinoma

Opis linii komórkowej

Derived from a 58 year old Caucasian male. The cells can synthesise lecithin utilising the cytidine diphosphocholine pathway. Occasional cells may also contain inclusion bodies although they are not known to carry any human pathogen.

Zastosowanie

Enzymatic studies

Human A549 cell line has been used:

• to study the ability of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-one (BChTT) to inhibit cancer cell proliferation and identify mechanisms involved in its actions
• to study the effects of insulin and insulin-like growth factor 1 (IGF1) on proliferation and apoptosis in A549 cells
• to culture A549 cells in different media over a long term and identify conditions for promoting alveolar type II differentiation characteristics in A549 cells

Profil DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 11
D7S820: 8,11
THO1: 8,9.3
TPOX: 8,11
vWA: 14

pożywka hodowlana

Ham′s F12K + 2mM Glutamine + 10% Foetal Bovine Serum FBS / FCS.

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or tTrypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Ta strona może zawierać tekst przetłumaczony maszynowo.