D5030

Dulbecco′s Modified Eagle′s Medium

Name: Dulbecco′s Modified Eagle′s Medium
Brand: SIGMA

Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture

Synonim(y):

DME, DMEM

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About This Item

Kod UNSPSC:

12352207

NACRES:

NA.75

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Pomoc techniczna

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Poziom jakości

500

Formularz

powder

metody

cell culture | mammalian: suitable

komponenty

phenol red: no
NaHCO₃: no
L-glutamine: no
HEPES: no
glucose: no
sodium pyruvate: no

Warunki transportu

ambient

temp. przechowywania

2-8°C

Opis ogólny

Dulbecco′s Modified Eagle′s Medium (DME) is a modification of Basal Medium Eagle (BME) that has been formulated with a 4-fold higher concentration of amino acids and vitamins. It includes additional supplementary components. The DME formula, first reported for culturing embryonic mouse cells, contained 1,000 mg/L of glucose. Modifying the medium with 4,500 mg/L glucose is optimal for culturing certain cell types.

The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of DME, and have the flexibility to optimize the formula for their own application.

Zastosowanie

Dulbecco′s Modified Eagle′s Medium has been used:

to culture the isolated flexor digitorum brevis (FDB) fibers to measure exogenous fatty acid (FA) utilization as part of oxygen consumption rate (OCR) measurements
to culture the isolated tibialis anterior (TA) muscle fibers for lactate measurements
to culture the human bone marrow mesenchymal stem cells for osteogenic differentiation to prepare a low-glucose medium to culture human hepatocellular carcinoma HepG2 cells