F0291

Fibroblast Growth Factor-Basic, human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonim(y): FGF2, Podstawowy ludzki czynnik wzrostu fibroblastów, bFGF, hBFGF
• CAS Number: 106096-93-9
• Numer MDL: MFCD00081382
• Kod UNSPSC: 12352202
• NACRES: NA.77

Właściwości

• Product Name: hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture
• pochodzenie biologiczne: human
• Poziom jakości: 200
• rekombinowane: expressed in E. coli
• Próba: ≥97% (SDS-PAGE)
• Formularz: lyophilized powder
• siła działania: ≤-0.6 ng/mL
• masa cząsteczkowa: 16.0 kDa
• opakowanie: pkg of 4X25 μg; pkg of 25 μg
• warunki przechowywania: avoid repeated freeze/thaw cycles
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: ≤1.00 EU/μg
• kolor: white to faint yellow cast
• rozpuszczalność: water: soluble 0.025 mg, clear, colorless to faintly yellow
• numer dostępu UniProt: P09038
• temp. przechowywania: −20°C
• informacje o genach: human ... FGF2 (2247)

Opis

Opis ogólny

Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.

Zastosowanie

Fibroblast growth factor-basic (FGF-Basic) is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines, such as mesenchymal stem (stroma) cells (MSC).

Postać fizyczna

Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.

Komentarz do analizy

The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)