F3165

Monoclonal ANTI-FLAG^® M2 antibody produced in mouse

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

• Synonim(y): Anti-ddddk, Anti-dykddddk
• Kod UNSPSC: 12352203
• NACRES: NA.32

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• białko sprzężone: unconjugated
• forma przeciwciała: purified immunoglobulin (Purified IgG1 subclass)
• rodzaj przeciwciała: primary antibodies
• klon: M2, monoclonal
• Formularz: buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
• oczyszczone przez: using Protein A
• reaktywność gatunkowa: all
• stężenie: 3.8-4.2 mg/mL
• metody: western blot: 10 μg/mL (Protein A)
• izotyp: IgG1
• sekwencja immunogenna: DYKDDDDK
• Warunki transportu: dry ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Anti-Flag M2 antibody is used for the detection of Flag fusion proteins. This monoclonal antibody is produced in mouse and recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site. M2, unlike M1 antibody is not Calcium dependent.
F3165 is affinity purified using Protein A resin, so it contains not only the anti-FLAG M2 antibody but also small amounts of native mouse IgG, increasing its sensitivity in most applications.
Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Zastosowanie

Monoclonal ANTI-FLAG^® M2 antibody produced in mouse has been used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunofluorescence
• ELISA
• EIA
• chromatin immunoprecipitation
• electron microscopy
• flow cytometry
• supershift assays

Browse additional application references in our FLAG^® Literature portal.

Uwaga dotycząca przygotowania

Dilute the antibody solution from 0.5-10 ug/mL in Tris Buffered Saline, pH 8.0, with 3% nonfat milk

Przechowywanie i stabilność

Store the undiluted antibody at –20 °C in working aliquots. Repeated freezing and thawing is not recommended.
Note: Overtime, small amounts of purified antibodies can precipitate from solution due to intermolecular hydrophobic interactions. If a precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in Western blot or immunoprecipitation applications.

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Informacje prawne

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable