L1882

Lectin from Triticum vulgaris (wheat)

agarose conjugate, saline suspension

• Synonim(y): WGA, Wheat germ agglutinin
• Kod UNSPSC: 12352202
• NACRES: NA.32

Właściwości

• białko sprzężone: agarose conjugate
• Poziom jakości: 200
• Formularz: saline suspension
• zakres etykietowania: 5-10 mg per mL
• Matryca: cross-linked 4% beaded agarose
• aktywacja macierzy: cyanogen bromide
• pojemność: ≥0.1 mg/mL binding capacity (ovomucoid)
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Zastosowanie

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)₂ or N-acetylneuraminic acid.

Działania biochem./fizjol.

Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Postać fizyczna

Suspension in 1.0 M NaCl containing 15 mM sodium azide

Uwaga dotycząca przygotowania

Conjugates are prepared from affinity purified lectin.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Środki ochrony indywidualnej: Eyeshields, Gloves