L3892

Lectin from Triticum vulgaris (wheat)

peroxidase conjugate, lyophilized powder

• Synonim(y): WGA, Wheat germ agglutinin
• Kod UNSPSC: 12352202
• NACRES: NA.32

Właściwości

• pochodzenie biologiczne: Triticum vulgaris
• Poziom jakości: 200
• białko sprzężone: peroxidase conjugate
• Próba: ≥85% protein basis (Warburg-Christian)
• Formularz: lyophilized powder
• siła działania: <40 μg/mL agglutination activity
• peroxidase activity: 50-200 units/mg protein
• skład: Protein, ~90% modified Warburg-Christian
• temp. przechowywania: −20°C

Opis

Zastosowanie

Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

Działania biochem./fizjol.

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Opakowanie

Package size based on protein content.

Definicja jednostki

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Postać fizyczna

Contains citrate buffer

Uwaga dotycząca przygotowania

Conjugates are prepared from affinity purified lectin.

Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)