M3769

Medium 199

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

• Synonim(y): M199 Medium, TCM 199
• Kod UNSPSC: 12352207
• NACRES: NA.71

Właściwości

• Poziom jakości: 500
• Formularz: powder
• metody: cell culture | mammalian: suitable
• komponenty: L-glutamine: no; phenol red: no; HEPES: no; sodium pyruvate: no; Earle’s salts (5% CO₂): yes; NaHCO₃: no
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Zastosowanie

Medium 199 has been used:

• to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
• to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
• to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)

Ilość

Formulated to contain 9.4 grams of powder per liter of medium.

Rekonstytucja

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable