M9309

McCoy′s 5A Medium

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• Synonim(y): Podłoże podstawowe 5A
• Numer MDL: MFCD00217560
• Kod UNSPSC: 12352207
• NACRES: NA.71

Właściwości

• Product Name: McCoy′s 5A Medium, Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• Poziom jakości: 300
• sterylność: sterile-filtered
• Formularz: liquid
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: endotoxin, tested
• komponenty: NaHCO₃: 2.2 g/L; L-glutamine: 0.21 g/L; phenol red: 0.011 g/L; glucose: 3.0 g/L (Dextro)
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

The amino acid requirements for in vitro cultivation of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.

Zastosowanie

McCoy′s 5A Medium has been used:

• to culture HCT-116?cells
• to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1
• to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies

Działania biochem./fizjol.

McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable