N3520

Nutrient Mixture F-12 Ham

Name: Nutrient Mixture F-12 Ham
Brand: SIGMA

Kaighn′s Modification, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonim(y):

Ham’s F-12

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About This Item

Numer MDL:

MFCD00217417

Kod UNSPSC:

12352207

NACRES:

NA.75

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Formularz

powder

metody

cell culture | mammalian: suitable

komponenty

NaHCO₃: no
sodium pyruvate: 0.22 g/L
HEPES: no
L-glutamine: yes
phenol red: yes

Warunki transportu

ambient

temp. przechowywania

2-8°C

Opis ogólny

The nutrient mixture Ham′s F-12 is developed by Ham and it has higher levels of amino acids, vitamins and trace elements. Putrescine and linoleic acid are added to the formulation. Nutrient medium was originally designed for the serum-free growth of Chinese hamster ovary and lung cells. When used with whole or dialyzed serum or in combination with hormones and transferrin, it is widely used to grow mammalian and hybridoma cells.

Zastosowanie

Nutrient Mixture F-12 Ham is used for the following applications:

HeLa (human cervix adenocarcinoma cells) were grown in this medium (nutrient mixture F-12 Ham) supplemented with 10% heat-inactivated fetal calf serum
Used in cell culture for different cells during maintenance and incubation (i.e. A549 cell line was maintained as a monolayer in nutrient mixture F-12 HAM Medium along with other components)
Used in media for the propagation of Cowdria ruminantium
Used during preparation of primary mixed glial cultures

Kaighn′s modification of Ham′s F-12 and Coon′s F-12 has increased concentrations of amino acids and pyruvate, as well as modified salts (Konigsbergs). This medium was designed to support the growth of differentiated rat and chicken cells, and primary human liver cells.