P7582

Amino-terminal FLAG-BAP^™ Fusion Protein

• Synonim(y): Amino-końcowe białko fuzyjne, Białko fuzyjne BAP, Białko fuzyjne FLAG-BAP
• Numer MDL: MFCD00130921
• Kod UNSPSC: 12352202
• NACRES: NA.32

Właściwości

• Formularz: liquid
• Poziom jakości: 200
• masa cząsteczkowa: ~49 kDa
• Warunki transportu: dry ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Amino-terminal FLAG-BAP^™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP^™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.

The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.

Zastosowanie

Amino-terminal FLAG-BAP^™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification and in western blotting based quantification of Flag-Wnt8 and Drosophila melanogaster transcription factor interactome.

Learn more product details in our FLAG^® application portal.

Inne uwagi

Control protein

Postać fizyczna

Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.

Informacje prawne

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)