P8804

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)

• Synonim(y): 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
• CAS Number: 37288-19-0
• Numer WE: 4.6.1.13 (BRENDA, IUBMB)
• Numer WE: 232-638-2
• Numer MDL: MFCD00131896
• Kod UNSPSC: 12352204
• NACRES: NA.32

Właściwości

• pochodzenie biologiczne: Bacillus sp. (Bacillus cereus)
• Poziom jakości: 200
• Formularz: buffered aqueous glycerol solution
• aktywność właściwa: ≥1,000 units/mg protein (Lowry)
• masa cząsteczkowa: 28 kDa
• obecność zanieczyszczeń: Phopholipase C (lecithinase) ≤1 units/mg protein; Sphingomyelinase ≤40 units/mg protein
• temp. przechowywania: 2-8°C
• informacje o genach: Bacillus cereus E33L ... BCZK3513(3026815)

Opis

Opis ogólny

Phospholipase C (PI-PLC) from Bacillus cereus has irregular triosephosphate isomerase (TIM)-barrel structure with eight-standard parallel β barrel. It is a 28 kDa protein.

Zastosowanie

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:

• in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)
• to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membrane
• to cleave immunolabeled HeLa cells

Działania biochem./fizjol.

Phospholipase C (PI-PLC) releases diacylglycerol by cleaving the glycosylphosphatidylinositol. It has broad substrate specificity and its activity is influenced by metal ions and surfactants.

Used for the release of GPI anchored proteins from the membrane.

Definicja jednostki

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

Postać fizyczna

Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA

Komentarz do analizy

Acetylcholinesterase is measured according to Ellman, et al.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)