SAE0091

Lysostaphin from Staphylococcus staphylolyticus

free of DNA contaminants, ≥500 units/mg protein, lyophilized powder, suitable for Microbiome research

• Synonim(y): Lysostaphin from Staphylococcus staphylolyticus, Glycyl-glycine Endopeptidase
• CAS Number: 9011-93-2
• Numer WE: 3.4.24.75 (BRENDA, IUBMB)
• Numer MDL: MFCD00131556
• Kod UNSPSC: 12352202
• NACRES: NA.54

Właściwości

• Product Name: Lysostaphin from Staphylococcus staphylolyticus, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥500 units/mg protein
• pochodzenie biologiczne: microbial (Staphylococcus staphylotyticus)
• Formularz: lyophilized powder
• aktywność właściwa: ≥500 units/mg protein
• masa cząsteczkowa: 27 kDa
• Właściwości: DNA free
• Warunki transportu: wet ice
• temp. przechowywania: −20°C

Opis

Zastosowanie

Purified extracellular lysostaphin from S. staphylolyticus SAE0091 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Lysostaphin is a zinc metalloenzyme isolated from a bacterial culture of Staphylococcus staphylolyticus. It has specific lytic action against other Staphylococcus species, including S. aureus.1,2 Lysostaphin has
hexosaminidase, amidase, and endopeptidase activities. It cleaves polyglycine crosslinks in the cellular wall which leads to cell lysis of Staphylococcus species, but not of other bacterial genera. [Pharmaceuticals 2010, 3(4), 1139-1161]
Lysostaphin is a single polypeptide chain of 246 amino acids, a molecular mass of 26,926 Da, isoelectric point of 9.5, (5) and an activity pH optimum of 7.5.(6)

Działania biochem./fizjol.

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic. pH Optimum for activity: ~7.5.

Definicja jednostki

One unit will reduce the turbidity (A620) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Postać fizyczna

Supplied as a lyophilized powder containing 50–70% protein with the balance primarily as NaCl.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable