SRE0082

Peroxidase from horseradish

Name: Peroxidase from horseradish
Brand: SIGMA

Synonim(y):

Peroxidase from horseradish, horse radish peroxidase, horseradish peroxidase, horseradish peroxidase (hrp), hrp, peroxidase, peroxidase enzyme, peroxidase from horseradish, Donor:hydrogen-peroxide oxidoreductase, Horseradish peroxidase

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About This Item

CAS Number:

9003-99-0

Numer WE:

1.11.1.7 (BRENDA, IUBMB)

Numer WE:

232-668-6

Numer MDL:

MFCD00071339

Kod UNSPSC:

12352204

NACRES:

NA.54

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opis

For research or further manufacturing use only. Not intended for direct use in humans or animals. Non-TSCA use only in US.

Poziom jakości

400

aktywność właściwa

≥250 units/mg solid (using pyrogallol)

przydatność

suitable for immunoassay
suitable for manufacturing use

Zastosowanie

diagnostic assay manufacturing

Warunki transportu

wet ice

temp. przechowywania

2-8°C

ciąg SMILES

[O+H2]O[O-]

InChI

1S/H2O3/c1-3-2/h1-2H

Klucz InChI

JSPLKZUTYZBBKA-UHFFFAOYSA-N

Opis ogólny

Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). HRP is a single chain polypeptide containing four disulfide bonds. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes is 3.0 - 9.0.

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca²⁺ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Zastosowanie

HRP is used in immunoassays, such as ELISAs, Western blots, and lateral flow assays. HRP is used in academic research, diagnostics, and pharmaceutical research. For more applications, see the following technical article: An Introduction to Horseradish Peroxidase (HRP) and Its Applications.

Peroxidase from horseradish has been used in western blotting.

Definicja jednostki

One pyrogallol unit will form 1.0 mg purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Komentarz do analizy

The RZ (Reinheitszahl) is the absorbance ratio A₄₀₃/A₂₇₅ determined at 0.5-1.0 mg/ml in deionized water. It is a measure of hemin content, not enzymatic activity. Even preparations with high RZ may have low enzymatic activity.