SRP3156

Sonic Hedgehog human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonim(y): HHG-1
• Kod UNSPSC: 12352200
• NACRES: NA.32

Właściwości

• pochodzenie biologiczne: human
• rekombinowane: expressed in E. coli
• Próba: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Formularz: lyophilized
• siła działania: 0.8-1.0 μg/mL ED₅₀
• masa cząsteczkowa: 20.0 kDa
• opakowanie: pkg of 25 μg
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: <0.1 EU/μg endotoxin, tested
• kolor: white
• numer dostępu UniProt: Q15465
• Warunki transportu: wet ice
• temp. przechowywania: −20°C
• informacje o genach: human ... SHH(6469)

Opis

Opis ogólny

The sonic hedgehog protein is encoded by the SHH gene that is mapped to human chromosome 7q36. It is homologous to the Drosophila segment polarity gene hedgehog. The encoded protein is a 45kDa precursor protein that is auto-cleaved to form SSH-N (an amino-terminal 20kDa product) and SSH-C (carboxy-terminal product). SSH-N is found to be cell-associated, while SSH-C diffuses freely. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring chemically modified Cys residue.

Zastosowanie

Sonic Hedgehog human has been used:

• To study its role in liver cancer stem cell activity.
• For neuronal differentiation.
• For cholinergic-like neurons (ChLNs) differentiation.

The sonic hedgehog protein is used in neuronal induction medium as a supplement.

Działania biochem./fizjol.

The SHH (sonic hedgehog) gene encodes a soluble signaling protein that functions in the patterning of cell differentiation in the neural tube and limb bud. It has also been implicated in several processes, such as developing nervous system, including midbrain and ventral forebrain neuronal differentiation and neuronal precursor proliferation. In adults, it regulates progenitor function and proliferation. Mutations in this gene have been associated with holoprosencephaly, a developmental defect of the forebrain and midface.

Sekwencja

IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Postać fizyczna

Lyophilized with no additives.

Rekonstytucja

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable