T9003

Trypsin inhibitor

Name: Trypsin inhibitor
Brand: SIGMA

lyophilized powder

Synonim(y):

SBTI

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About This Item

CAS Number:

9035-81-8

Numer WE:

232-906-9

Numer MDL:

MFCD00082103

Kod UNSPSC:

12352204

NACRES:

NA.54

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Product Name

Trypsin inhibitor from Glycine max (soybean), lyophilized powder

pochodzenie biologiczne

Glycine max (soybean)

Poziom jakości

300

Formularz

lyophilized powder

masa cząsteczkowa

20.1 kDa

rozpuszczalność

H₂O: >10 mg/mL
phosphate buffer: >10 mg/mL (hazy above 10 mg/ml)
soluble

Zastosowanie

diagnostic assay manufacturing

temp. przechowywania

2-8°C

Zastosowanie

Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin.

Działania biochem./fizjol.

This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.

Komponenty

The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.

Definicja jednostki

One trypsin unit = A₂₅₃ of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Uwaga dotycząca przygotowania

The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet.

Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.