IMR-90 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85020204, human lung (fetal), Fibroblast

• Synonym(s): I90 Cells, IMR90 Cells, Institute for Medical Research-90 Cells
• UNSPSC Code: 41106514

Properties

• biological source: human lung (fetal)
• description: Human Caucasian fetal lung fibroblast
• packaging: tube of 5 μg 85020204-DNA-5UG; pkg of vial of cells 85020204-1VL
• growth mode: Adherent
• karyotype: 2n = 46, diploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian foetal lung fibroblast

Cell Line Description

Derived from lung tissue of a 16 week old female Caucasian foetus. The cells have a virus susceptibility similar to WI-38 (Sigma catalogue no. 90020107) and MRC-5 cells (Sigma catalogue no. 84101801). The cells have a finite lifespan. Available as a growing order only.

Application

IMR-90 human cell line has been used to study the cytotoxicity of hydroxyapatite/gold/arginine (HAp/Au/arginine) nanoparticles.

Virus studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,9
vWA: 16,19

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.