O1516

Oil Red O solution

0.5% in propylene glycol

• Synonym(s): Solvent Red 27, Sudan Red 5B
• Empirical Formula (Hill Notation): C₂₆H₂₄N₄O
• CAS Number: 1320-06-5
• Molecular Weight: 408.49
• UNSPSC Code: 12171500
• NACRES: NA.47

Properties

• form: liquid
• Quality Level: 200
• concentration: 0.5% in propylene glycol
• technique(s): microbe id | staining: suitable
• λmax: 0.40-1.00 at 506-520 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: N(\N=C3\c4c(cccc4)C=CC\3=O)c1c(cc(c(c1)C)N=Nc2c(ccc(c2)C)C)C
• InChI: 1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-
• InChI key: HJAYEODIXUYVIC-LTFAAIOASA-N

Description

General description

Oil Red O stains and imparts red-orange color to lipids. It has also been shown to mark vacuoles, observed in acute lymphoblastic leukemia (ALL-L3) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Application

Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Oil red O solution has been used to identify intracytoplasmatic lipid droplets on 3T3-L1 cell line adipocytes and to measure lipid content in Simpson-Golabi-Behmel syndrome (SGBS) preadipocytes.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 217.4 °F
• Flash Point(C): 103 °C