V900497

Pepsin from porcine gastric mucosa

Vetec^™, reagent grade

• Synonym(s): Pepsin A, Pepsin from hog stomach
• CAS Number: 9001-75-6
• EC Number: 3.4.23.1 (BRENDA, IUBMB)
• EC Number: 232-629-3
• MDL number: MFCD00081840
• UNSPSC Code: 12352204

Properties

• grade: reagent grade
• product line: Vetec^™
• form: solid
• specific activity: 400-800 units/mg protein
• mol wt: 35 kDa

Description

Application

Pepsin cleavage can be used to produce F(ab′)₂ fragments of antibodies. pepsin at www.sigma-aldrich.com/enzymeexplorer.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Unit Definition

One unit will produce a change in A280 of 0.001 per minute at pH 2.0 at 37 deg C, measured as TCA-Soluble products using hemoglobin as substrate.

Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.

Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable