A3312

Anti-Human IgG (γ-chain specific), F(ab′)₂ fragment−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

• MDL number: MFCD00162443
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: goat
• Quality Level: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• species reactivity: human
• technique(s): direct ELISA: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50; western blot: 1:30,000
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Anti-Human IgG (γ-chain specific), (F(ab′)₂) fragment-Alkaline Phosphatase antibody is specific for human IgG when tested against human IgA, IgG, IgM, and Bence Jones κ and λ myeloma proteins.

Immunogen

Purified human IgG

Application

Goat anti-human IgG (gamma-chain specific), F(ab′)2 fragment-alkaline phosphatase antibody can be used for western blot (1:30,000) applications.

Anti-Human IgG (γ-chain specific), (F(ab′)2) fragment-Alkaline Phosphatase antibody is suitable for use in ELISA and immunohistochemistry.

Serum IgGs against HHV-6 or HHV-7 were detected by Elisa using alkaline phosphatase conjugated goat anti-human IgG F′ab specific at a concentration of 1:1000 diluted in PBS/0.05% skim milk. The antibody was incubated on plates for 2 hours at 37 degrees.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl₂, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide as preservative

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves