230162
Sulforhodamine B
Dye content 75 %
Synonym(s):
Acid Red 52, Sulforhodamine B monosodium salt
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About This Item
Empirical Formula (Hill Notation):
C27H29N2NaO7S2
CAS Number:
Molecular Weight:
580.65
Colour Index Number:
45100
Beilstein/REAXYS Number:
3886008
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
assay
≥73% (HPLC)
Quality Level
form
powder
composition
Dye content, 75%
technique(s)
microbe id | staining: suitable
color
brown to very dark brown
green to very dark green
solubility
methanol: 1 mg/mL
λmax
554 nm
ε (extinction coefficient)
≥85000 at 554-560 nm in 0.1 M NaOH in methanol at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
Related Categories
General description
Sulforhodamine B (SRB) is a bright-pink aminoxanthene dye. The sulforhodamine B (SRB) assay is extensively used for in vitro cytotoxicity screening. SRB binds to the protein constituents of the cells in a stoichiometric manner. Thus, the amount of dye extracted from stained cells is directly proportional to the cell mass. SRB can also be used for cell density determination.
Application
Sulforhodamine B has been used as a dye in the MCF-7 (human breast adenocarcinoma cell line) cell proliferation assay.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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