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04-281

Sigma-Aldrich

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10

clone 5F10, Upstate®, from mouse

Synonym(s):

ERBB, ERBB1, epidermal growth factor receptor

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5F10, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG1

NCBI accession no.

NM_005228.3
NM_201282.1
NM_201283.1
NM_201284.1

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Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 clone 5F10, Upstate®, from mouse

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Anti-EGFR (CT) Antibody, clone 13G8 clone 13G8, from mouse

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Anti-EGFR (CT) Antibody, clone 13G8

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

5F10, monoclonal

clone

3F2, monoclonal

clone

12A3, monoclonal

clone

13G8, monoclonal

species reactivity

human

species reactivity

mouse, human, canine, rat

species reactivity

human, mouse

species reactivity

mouse, human

UniProt accession no.

P00533

UniProt accession no.

P00533

UniProt accession no.

P00533

UniProt accession no.

P00533

technique(s)

western blot: suitable

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, immunocytochemistry: suitable, western blot: suitable

Specificity

Recognizes EGFR when phosphorylated at Threonine 669.

Immunogen

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.

Application

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Quality

Routinely evaluated by immunoblot.

Target description

180 kDa

Physical form

100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.
Format: Purified
Thiophilic adsoption and size exclusion chromatography

Storage and Stability

6 months at -20°C from date of shipment

Analysis Note

Control
Includes PMA and pervanadate treated A431 cell lysate as a positive control

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

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