05-312

Anti-E2F-4 Antibody, clone GG22-2A6

clone GG22-2A6, Upstate^®, from mouse

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: GG22-2A6, monoclonal
• species reactivity: rat, mouse, human
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable (ChIP-seq); immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2b
• NCBI accession no.: NM_001950
• UniProt accession no.: Q16254
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... E2F4(1874)

Description

Specificity

E2F-4; does not recognize E2F-1, -2, -3, or -5

Immunogen

GST fusion to human E2F-4

Application

Detect E2F-4 with Anti-E2F-4 Antibody, clone GG22-2A6 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

routinely evaluated on non-stimulated A431 cell lysate

Target description

55kDa

Physical form

200μg of protein G purified mouse IgG_2b in 200μl of 0.07 M Tris-glycine, pH 7.4, 0.135 M NaCl, 0.035% sodium azide and 30% glycerol.

Format: Purified

Protein G Chromatography

Storage and Stability

1 year at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1