Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

05-366

Sigma-Aldrich

Anti-PLCγ-1 Antibody, clone B-6-4

clone B-6-4, 1 mg/mL, Upstate®

Sign Into View Organizational & Contract Pricing

Select a Size

All Photos(1)

Select a Size

Change View

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B-6-4, monoclonal

species reactivity

human, rabbit, rat, bovine, mouse

manufacturer/tradename

Upstate®

concentration

1 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

Specificity

PLCγ-1; may also react with some non-PLC γ-1 SH-3 containing proteins

Immunogen

Purified soluble phospholipase C (PLC) g-1 from bovine brain

Application

Anti-PLCγ-1 Antibody, clone B-6-4 is an antibody against PLCγ-1 for use in IP & WB.
Research Category
Signaling
Research Sub Category
Lipid Signaling

Quality

Routinely evaluated in immunoblot using acid precipitated protein from bovine brain, RIPA lysates from human A431 cells, and previously using mouse 3T3/A31 fibroblasts and rat L6 myoblasts lysates.

Target description

~135 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service