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Anti-PLCγ-1 Antibody, clone B-6-4

Name: Anti-PLCγ-1 Antibody, clone B-6-4
Brand: MM

clone B-6-4, 1 mg/mL, Upstate^®

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

Pricing and availability is not currently available.

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B-6-4, monoclonal

species reactivity

human, rabbit, rat, bovine, mouse

manufacturer/tradename

Upstate^®

concentration

1 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

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Show Differences

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This Item	HPA015006	HPA035434	ABS1013
SAB2500652 Anti-MTHFD1 antibody produced in goat	HPA015006 Anti-MTHFD1 antibody produced in rabbit	HPA035434 Anti-MTHFD2L antibody produced in rabbit	ABS1013 Anti-MTHFR Antibody
biological source goat	biological source rabbit	biological source rabbit	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate -
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form serum
shipped in dry ice	shipped in wet ice	shipped in wet ice	shipped in wet ice
technique(s) indirect ELISA: suitable, western blot: suitable	technique(s) immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:200-1:500	technique(s) immunohistochemistry: 1:20- 1:50	technique(s) western blot: suitable

Specificity

PLCγ-1; may also react with some non-PLC γ-1 SH-3 containing proteins

Immunogen

Purified soluble phospholipase C (PLC) g-1 from bovine brain

Application

Anti-PLCγ-1 Antibody, clone B-6-4 is an antibody against PLCγ-1 for use in IP & WB.

Research Category
Signaling

Research Sub Category
Lipid Signaling

Quality

Routinely evaluated in immunoblot using acid precipitated protein from bovine brain, RIPA lysates from human A431 cells, and previously using mouse 3T3/A31 fibroblasts and rat L6 myoblasts lysates.

Target description

~135 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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ZRB1774

Anti-PLCγ1 Antibody, clone 1H21 ZooMAb^® Rabbit Monoclonal, recombinant, expressed in HEK 293 cells

pictograms

GHS07

signalword

Warning

hcodes

H302 + H312 + H332,H412

pcodes

P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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SH2-B is required for nerve growth factor-induced neuronal differentiation.

L Rui et al.

The Journal of biological chemistry, 274(15), 10590-10594 (1999-04-03)

Nerve growth factor (NGF) is essential for the development and survival of sympathetic and sensory neurons. NGF binds to TrkA, activates the intrinsic kinase activity of TrkA, and promotes the differentiation of pheochromocytoma (PC12) cells into sympathetic-like neurons. Several signaling

T cell activation-dependent association between the p85 subunit of the phosphatidylinositol 3-kinase and Grb2/phospholipase C-gamma 1-binding phosphotyrosyl protein pp36/38.

T Fukazawa et al.

The Journal of biological chemistry, 270(34), 20177-20182 (1995-08-25)

Tyrosine phosphorylation of cellular proteins is an early and an essential step in T cell receptor-mediated lymphocyte activation. Tyrosine phosphorylation of transmembrane receptor chains (such as zeta and CD3 chains) and membrane-associated proteins provides docking sites for SH2 domains of

Phospholipase C and src tyrosine kinases mediate neurotensin-stimulated Cl- secretion in rabbit proximal colon.

Roli Prasad, Jayashree Venkatasubramanian, Milen Amde, Mrinalini Chatta Rao

Digestive Diseases and Sciences null

Tyrosine phosphorylation and recruitment of ZAP-70 to the CD3-TCR complex are defective after CD2 stimulation

Hubert, P., et al

Journal of immunology (Baltimore, Md. : 1950), 157, 4322-4332 (1996)

CD3 stimulation causes phosphorylation of phospholipase C-gamma 1 on serine and tyrosine residues in a human T-cell line.

Park, D J, et al.

Proceedings of the National Academy of Sciences of the USA, 88, 5453-5456 (1991)

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