1.07599

Rhodamine B (C.I.45170)

for microscopy

• Synonym(s): Rhodamine B (C.I.45170), Tetraethylrhodamine, Brilliant pink B
• Empirical Formula (Hill Notation): C₂₈H₃₁ClN₂O₃
• CAS Number: 81-88-9
• Molecular Weight: 479.01
• MDL number: MFCD00011931
• UNSPSC Code: 41116130
• EC Index Number: 201-383-9

Properties

• Quality Level: 400
• form: solid
• potency: >2000 mg/kg LD₅₀, oral (Rat)
• composition: Dye, ≥90% spectrophotometric assay
• pH: 3-4 (20 °C, 10 g/L in H₂O)
• mp: 199-201 °C
• solubility: 15 g/L
• density: 1.31 g/cm³ at 20 °C
• bulk density: 250 kg/m³
• εmax: 2115-2350 at 550-552 nm in 50% ethanol at 0.003 g/L

Description

General description

Rhodamine B (C.I. 45170) for microscopy, is a solid dye for fluorescence AFB staining of bacteriological smears and histological tissue sections of human origin.The mechanism is a non-specific binding of fluorescence dye on a substrate, similar to the classical Ziehl-Neelsen method, although the heating of the Rhodamine B color solution can be avoided. The dye is a CE certified IVD product and has a constant dye content and a reliable high quality, which is one of the major precondition for a reproducible diagnostic staining. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 90 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 550 - 552 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 2115 - 2350
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test

Safety Information

• pictograms: GHS05,GHS07
• signalword: Danger
• hcodes: H302 - H318 - H412
• pcodes: P264 - P270 - P273 - P280 - P301 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_c: Not applicable