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14-452-M

Sigma-Aldrich

PDK1 Protein, active, 10 µg

Active, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.26
Pricing and availability is not currently available.

biological source

human

Quality Level

100

recombinant

expressed in baculovirus infected Sf21 cells

product line

Upstate®

specific activity

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)

mol wt

Mw 59 kDa

purified by

(Ni2+/NTA-agarose)

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

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PDK1 Protein, active, 10 µg Active, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.

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PDK1 Protein, active, 10 µg

Plk1 Protein, active, 10 µg Active, N-terminal 6His tagged, recombinant, human Plk1. For use in Kinase Assays.

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PAK1 Protein, active, 10 µg Active, N-terminal FLAG-tagged, recombinant, human PAK1, amino acids 150-end. For use in Kinase Assays.

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PAK1 Protein, active, 10 µg

Cdk1/cyclin B Protein, active, 10 µg Active, C-terminal His6-tagged human full length Cdk1 & N-terminal GST-tagged human full length Cyclin B, for use in Kinase Assays.

14-450-M

Cdk1/cyclin B Protein, active, 10 µg

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

technique(s)

activity assay: suitable (kinase)

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

-

biological source

human

biological source

human

biological source

human

biological source

human

mol wt

Mw 59 kDa

mol wt

Mw 69.3 kDa

mol wt

Mw 46 kDa

mol wt

Mw 35 kDa (cdk1), Mw 75 kDa (cyclin B)

Gene Information

human ... PDPK1(5170)

Gene Information

-

Gene Information

-

Gene Information

human ... CCNB1(891), CDK2(891)

specific activity

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)

specific activity

-

specific activity

-

specific activity

-

General description

N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end.

Biochem/physiol Actions

Protein Target: PDK1
Target Sub-Family: AGC

Packaging

Also available in 10 μg size and in bulk
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-452M when ordering the 250 µg size.

Quality

Routinely evaluated by phosphorylation of PDKtide

Target description

PDK1

Physical form

50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 0.1% 2-mercaptoethanol, 1mM benzamidine, 200μM PMSF and 5% glycerol
Ni2+/NTA-agarose

Storage and Stability

6 months at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_c

Not applicable

  • Specification Sheet

    • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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