14-604M-D

PI3 kinase (p110δ/p85α)

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Properties

• assay: ≥75%
• Quality Level: 100
• specific activity: 500 pmol/min-μg
• technique(s): activity assay: suitable
• UniProt accession no.: O00329
• Gene Information: human ... PIK3CD(5293)

Description

General description

Complex of N-terminal GST recombinant full-length human p110δ, and recombinant full length, human p85α, coexpressed in a Baculovirus infected Sf9 cell expression system

Quality

Evaluated by Kinase assay: A 25 μl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.5), 20 mM MgCl2, 100 uM ATP, 200 μM PI:PS substrate and p110δ/p85α for 17 minutes at 30°C. ATP reduction is detected using ADP-Glo Kinase Assay Platform

Physical properties

146 kDa (p110δ), 84 kDa (p85α)

Physical form

25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.03% Tween-20, 3 mM DTT, and 50% glycerol

Storage and Stability

Stable for > 6 month at –80°C from date of receipt.

Other Notes

SIZE: 1044 amino acids; 119479 Da
SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Interacts with ERAS (By similarity).
TISSUE SPECIFICITY: Expressed predominantly in leukocytes.
PTM: Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity.
SIMILARITY: SwissProt: O00329 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.