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14-811

Sigma-Aldrich

UbcH5c Conjugating Enzyme, 100 µg

The His6-tagged fusion protein of UbcH5c is charge & support ubiquitinylation in vitro. The His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions.

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.32
Pricing and availability is not currently available.

biological source

human

Quality Level

100

assay

>95% (SDS-PAGE)

mol wt

Mw 17 kDa

manufacturer/tradename

Upstate®

NCBI accession no.

NM_003340.4
NM_181886.1
NM_181887.1
NM_181888.1
NM_181889.1
NM_181890.1
NM_181891.1
NM_181892.1
NM_181893.1

UniProt accession no.

P61077

shipped in

dry ice

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manufacturer/tradename

Bel-Art F37874-0005

manufacturer/tradename

Bel-Art F37874-0001

manufacturer/tradename

Bel-Art F37874-1020

manufacturer/tradename

Bel-Art F37874-0010

material

high-density polyethylene

material

-

material

high-density polyethylene

material

high-density polyethylene

packaging

pack of 20 ea (individually wrapped)

packaging

pack of 20 ea (individually wrapped)

packaging

pack of 20 ea (individually wrapped)

packaging

pack of 20 ea (individually wrapped)

capacity × L

5 mL (0.17 oz) × 19.75 in. (50 cm)

capacity × L

1 mL (0.03 oz) × 19.75 in. (50 cm)

capacity × L

20 mL (0.67 oz) × 39.375 in. (100 cm)

capacity × L

10 mL (0.34 oz) × 19.75 in. (50 cm)

General description

Product Source: Recombinant human UbcH5c Conjugating Enzyme expressed in E. coli.
The His6-tagged fusion protein of UbcH5c is charge and support ubiquitinylation in vitro. Unlike their GST-tagged counterparts, the His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation. UbcH5c is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53, NF-κB, and c-Fos. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions.

Storage and Stability

Stable for 1 year at -80°C from date of shipment. Avoid multiple freeze/thaw cycles. After initial defrost, aliquot into individual tubes and refreeze at -80°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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    O Coux et al.
    The Journal of biological chemistry, 273(15), 8820-8828 (1998-05-16)
    Nuclear factor kappaB1 (NF-kappaB) is a heterodimeric complex that regulates transcription of many genes involved in immune and inflammatory responses. Its 50-kDa subunit (p50) is generated by the ubiquitin-proteasome pathway from a 105-kDa precursor (p105). We have reconstituted this proteolytic
    J P Jensen et al.
    The Journal of biological chemistry, 270(51), 30408-30414 (1995-12-22)
    Two very closely related human E2 ubiquitin conjugating enzymes, UbfH5B and UbcH5C, have been identified. These enzymes are products of distinct genes and are 88-89% identical in amino acid sequence to the recently described human E2, UbcH5 (now designated UbcH5A)

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