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208773

Sigma-Aldrich

Calpain Substrate IV, Cell-Permeable, Fluorogenic

The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

Synonym(s):

Calpain Substrate IV, Cell-Permeable, Fluorogenic

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About This Item

Empirical Formula (Hill Notation):
C127H206N50O27S
Molecular Weight:
2897.38
UNSPSC Code:
12352200
NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

100

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

solubility

DMSO: 5 mg/mL

fluorescence

λex 480 nm; λem 320 nm

shipped in

ambient

storage temp.

−20°C

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208771208772208748
Calpain Substrate IV, Cell-Permeable, Fluorogenic The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

208773

Calpain Substrate IV, Cell-Permeable, Fluorogenic

Calpain Substrate III, Fluorogenic A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2.

208771

Calpain Substrate III, Fluorogenic

Calpain-1 Substrate II, Fluorogenic An internally quenched peptide substrate that is optimized for calpain-1 amino acid recognition motifs at the primed as well as the non-primed sides.

208772

Calpain-1 Substrate II, Fluorogenic

Calpain-1 Substrate, Fluorogenic An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.

208748

Calpain-1 Substrate, Fluorogenic

assay

≥95% (HPLC)

assay

≥98% (HPLC)

assay

≥97% (HPLC)

assay

≥95% (HPLC)

form

lyophilized solid

form

lyophilized solid

form

lyophilized solid

form

lyophilized solid

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage condition

OK to freeze, protect from light, desiccated (hygroscopic)

storage condition

OK to freeze, protect from light

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

General description

The non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (kcat = 0.2 s-1, kcat/KM = 5000 M-1 s-1) when compared to the parental peptide (kcat = 0.17 s-1, kcat/KM = 680 M-1 s-1). Its suitability in detecting cellular Ca2+-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca2+ addition.
The non-permeant fluorogenic Calpain Substrate III is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂

Physical form

Supplied as a trifluoroacetate salt

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Banoczi, Z., et al. 2008. Bioconjug. Chem.19, 1375.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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