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CELLULYSIN™ Cellulase, Trichoderma viride

Synonym(s):

β-D-glucosidase, endo-β-1,4 glucanase, cellobiohydrolase

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About This Item

CAS Number:
9012-54-8
EC Number:
3.2.1.4 (BRENDA, IUBMB)
MDL number:
MFCD00081510
UNSPSC Code:
12352202
NACRES:
NA.54
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biological source

fungus (Trichoderma viride)

form

solid

specific activity

≥10,000 units/g dry wt.

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

technique(s)

transformation: suitable

color

yellow-white

solubility

water: soluble

suitability

suitable for molecular biology

application(s)

food and beverages
pharmaceutical

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Myoglobin from equine heart ≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

M1882

Myoglobin from equine heart

biological source

horse heart

biological source

Saccharomyces cerevisiae

biological source

-

biological source

equine heart

assay

≥95% (SDS-PAGE), ≥95% based on Mol. Wt. 12,384 basis

assay

≥85% based on Mol. Wt. 12,588 basis

assay

-

assay

≥90% (SDS-PAGE)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell based assay: suitable

technique(s)

mass spectrometry (MS): suitable

technique(s)

MALDI-MS: suitable

form

powder

form

powder

form

solid

form

essentially salt-free, lyophilized powder

suitability

suitable for molecular biology

suitability

-

suitability

-

suitability

-

mol wt

12,384

mol wt

12,588 Da

mol wt

(M+H+) 12,361.96 Da by calculation

mol wt

-

General description

Cellulase includes endo-β-1,4 glucanase, cellobiohydrolase, and β-D-glucosidase. It is commonly produced by bacteria and fungi.[1]

Application

CELLULYSIN™ Cellulase, Trichoderma viride has been used:
  • to digest transgenic BY-2 cells exposed to paraformaldehyde fixation[2]
  • in the enzyme mix used for in situ hybridization to plant chromosomes[3]
  • in the enzyme solution for the transformation of Phytophthora species[4]
  • to isolate intact cells and protoplasts from plant tissues

Biochem/physiol Actions

Cellulase is useful in food, feed, textile, leather, detergent, and paper industries. It is also applied in fiber modification, biomass fermentation, and pharmaceutical industries.[1]

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is a measure of decomposing activity using a filter paper substrate at 40°C, pH 4.0.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 month at -20°C.

Other Notes

Takebe, I., et al. 1968. Plant Cell Physiol.9, 115.
Tomita, Y., et al. 1968. J. Ferment. Technol.46, 701.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
CELLULYSIN is a trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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