567333

SNAPtide Botulinum Toxin A Substrate, Fluorogenic

≥90% (HPLC), lyophilized, FRET substrate, Calbiochem^®

• Synonym(s): Botulinum Toxin Assay
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Product Name: SNAPtide Botulinum Toxin A Substrate, Fluorogenic, SNAPtide Botulinum Toxin A Substrate, Fluorogenic, is a synthetic peptide that acts as a FRET substrate with the fluorophore o-aminobenzoic acid and the acceptor chromophore 2,4-dinitrophenol (DNP).
• Quality Level: 100
• assay: ≥90% (HPLC)
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• fluorescence: λ_ex ~326 nm; λ_em ~423 nm
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

A synthetic peptide, fluorescence resonance energy transfer (FRET) substrate containing the N-terminally-linked fluorophore o-aminobenzoic acid (Abz) and the acceptor chromophore 2,4-dinitrophenol (DNP) linked to the C-terminus. The substrate is based on the native botulinum toxin type A cleavage site found in the synaptosome-associated protein SNAP-25. Cleavage of the substrate by botulinum toxin releases the fluorophore and full fluorescence is restored.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Substrate for the native botulinum toxin

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from water.

Preparation Note

Prepare a 5 mM stock solution of this peptide in DMSO as follows: Add 40 µl of DMSO to a vial containing 200 nmol of peptide.

Reconstitution

Following reconstitution, aliquot, cover with foil to protect from light, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Bigalke, H. and Shoer, L.F. 2000. In: Handbook of Experimental Pharmacology,145, Bacterial Protein Toxins (K. Aktories and I. Just, Eds.) pp. 407-443, Springer-Verlag, Berlin.
Schiavo, G., et al. 1993. J. Biol. Chem.268, 23784.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable